Purification of membrane-bound galactosyltransferase from rat liver microsomal fractions
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چکیده
منابع مشابه
Purification and properties of microsomal UDP-glucuronosyltransferase from rat liver.
p-Nitrophenol conjugating activity associated with liver microsomal UDP-glucuronosyltransferase (EC 2.4.1.17) was purified 150- to 200-fold from cell-free homogenates. The purification scheme included solubilization with the nonionic detergent Lubrol WX, anion exchange chromatography at pH 6.0 and 7.5, and affinity chromatography with UDP-hexanolamine Sepharose 4B. The enzyme purified as a phos...
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The membrane fraction of a rat liver post-mitochondria1 supernatant is largely derived from endoplasmic reticulum, but also contains plasma-membrane fragments and Golgi membranes (see, e.g., Depierre & Dallner, 1975). The subfractionation of microsomal preparations normally depends on the use of high centrifugal fields, and hence large hydrostatic pressures. We have attempted to avoid these har...
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Microsomal epoxide hydrolase was purified from rat liver, and different fractions of the purified enzyme, which varied in their contents of phospholipid, were obtained by ion-exchange chromatography. One fraction (A), which did not bind to CM-cellulose, had a high phospholipid content, and a second fraction (B), which was eluted from CM-cellulose at high ionic strength, had a low phospholipid c...
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Some of the properties of galactosyltransferase of Golgi membrane-rich fractions from rat liver were investigated. The kinetic properties of the enzyme were determined from the initial rates of reaction under various conditions of incubation. Puromycyin, a known inhibitor of protein synthesis, was shown to inhibit the galactasyltransferase activity in vitro. The inhibition depended on the conce...
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Inrat liver microsomal fractions oxidation of adrenaline was effected by superoxide anion radicals (A), whereas cytochrome c, 2,6-dichlorophenol-indophenol and ferricyanide accepted electrons from NADPH-specific filavoprotein only directly. Nitro Blue Tetrazolium was reduced both by 02and by tbe direct acceptance of electrons. Elevation ofpH and addition of menadione shift the Nitro Blue Tetraz...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1977
ISSN: 0264-6021
DOI: 10.1042/bj1640541